Cartilage tissue engineering of TGF-β3 combined with IGF-1-induced bone marrow mesenchymal stem cells derived chondrocytes

VernacularTitle:骨髓间充质干细胞在β3转化生长因子联合胰岛素样生长因子-1定向诱导下的软骨组织工程
Author: Yulan LIU ; Jun REN ; Pei DENG
Publication Type:Journal Article
Keywords: Bone marrow mesenehymal stem cell; Alginate; Chondrogenesis; Cartilage tissue engineering
From: Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(2):122-126
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of IGF-1 on the TGF-β3 induced chondrogenesis of MSCs encapsulated in alginate beads and its application in cartilage tissue engineering.Methods MSC chondrogenesis in alginate beads was induced by TGF-β3 and/or IGF-1.Collagen type Ⅱ,aggrecan and Sox-9 expression was evaluated by immunostaining,RT-PCR and Western blot,respectively.Scanning electron microscope and laser confocal microscope were used to observe the differentiated chondrocytes when cultured on the chitosan-based scaffold.Results TGF-β3 with IGF-1 induced MSCs in alginate beads to express the higher level of collagen type Ⅱ,aggrecan and Sox-9 than any other growth factor alone (P＜0.05).The correlation coefficient between Sox9 and collagen type Ⅱ or aggrecan was 0.95 and 0.91,respectively.The chitosan-based scaffold supported the cell's adhesion,migration and proliferation.Conlusion IGF-1 enhances the TGF-β3-induced MSC chondrogenesis via upregulating Sox9 expression.The chitosan-based scaffold is biocompatible with the differentiated chondrocytes.