Clinical significance of the cytokines shift in patients of colonic cancer

VernacularTitle:大肠癌患者细胞因子的变化及其临床意义
Author: Lijuan QIAO ; Kerong MA ; Xianxia MAI ; Qing ZHAO ; Yaoping LI
Publication Type:Journal Article
Keywords: Intestinal neoplasms; Cytokines; Flow cytometry
From: Cancer Research and Clinic 2009;21(2):98-100
CountryChina
Language:Chinese
Abstract: Objective To observe the levels of Th1 and Th2 type cytokines of peripheral blood CD+4 T lymphocytes at different stage of treatment of colonic cancer,in order to find out the laboratory evidence of tumor immunotherapy.Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance the expression of cytokines.Then specific fluorescent-conjugated monoclonal antibodies were added to combine with its specific antigens.Cytokines were detected by flow cytometry.Results At different stage of treatment of colonic cancer,the levels of Th1 type cytokines of each stage was significant.The levels of Th2 type cytokines at each stage were not significant.The IFN-γ//IL-4 ratio were significantly different during tumor immunothempy.Conclusion At different stage of treatment of colonic cancer,the levels of Th,type cytokines were obviously associated with treatment.The levels of Th2 type cytokines had no association with treatment.Among them,the changes of Th1/Th2 ratio (IFN-γ/IL-4) had more correlation with tumor immunotherapy.So the reasonable immunotherapy can reverse Th1/Th2 ratio and immune inhibition.