Effects of verapamil and nicardipine on human sear fibroblast in serum-free culture
10.3760/cma.j.issn.1671-0290.2009.02.013
- VernacularTitle:维拉帕米及尼卡地平对瘢痕组织抑制作用的体外实验
- Author:
Hongtao YANG
;
Jianhong LIANG
;
Jie QI
;
Li YAN
;
Rui CAO
;
Yanjie LIU
;
Wen LI
;
Bingxin LU
;
Danqing DUAN
- Publication Type:Journal Article
- Keywords:
Verapamil;
Nicardipine;
Scar;
Fibroblast
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2009;15(2):118-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of verapamil and nicardipine on human scar fibroblast in serum-free culture and to compare the effectness of the two drugs.Methods We used MTT method to detect the effect of two drugs on human scar fibroblast proliferation:adding verapamil and nicardipine with different concentrations in the culture of fibroblasts which were in logarithmic growth phase (150,100,50,10,0μmol/L).After 24,72,and 120 h,we used MTT method to detect the cell proliferation,and converted the absorbance into growth inhibitory ratio.Results Verapamil and nicardipine showed the definite inhibition on the hypertrophic scar fibroblast (HSFB) and keloid fibroblast (KDFB) which were cultured in vitro.There was some difference in the action feature.In the earlier period,the effect of verapamil was powerful than that of nicardipine.With time,the effect did not reinforce.When fibroblast had been cultured for three to five days,the inhibition became weak.But nicardipine showed lasting inhibition on fibroblast proliferation.Conclusion Combination of verapamil with nicardipine may be a valuable method in the treatment of scar.
