Effects of silenced Racl on invasion and migration of LoVo cells
10.3760/cma.j.issn.0254-1432.2009.03.010
- VernacularTitle:沉默Racl基因对LoVo细胞侵袭移动的影响
- Author:
Shiyi ZHA
;
Zhenshu ZHANG
;
Yan SUN
;
Zhuosheng LAI
;
Qingzhen NAN
;
Kang LI
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Rho factor;
RNA interference;
Cytoskeleton Neoplasm invasiveness
- From:
Chinese Journal of Digestion
2009;29(3):173-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of silenced Racl on invasion and migration in LOVo cells.Methods The expression of Racl mRNA and protein in colorectal cancer cells(including LoVo SW480.SW620.SW1116,HT29)were detected by RT-PCR and Western blot,respectively.The changes of cytoskeleton were observed in LoVO cells after transfected with Racl-shRNA.then invasion and migration were recorded respectively in LoVo cells after transfected with Racl-N17 and Racl-L61.Results Racl mRNA and protein were overexpressed in all selected colorectal cancer cells.Deletion of Racl decreased the cross-linked actin network and pseudopodia,and inhibited the invasion and migration in LoVo cells.The migration experiment showed that the migrated cells were higher in Racl-shRNA[(75±5)cells].Racl-N17 [(93±5)cells]and Racl-L61[(267±7)cells]groups compared with control group[(214±8)cells,P＜0.01,＜O.01 and＜0.05,resprectively].The invasion experimental study revealed that the migrated cells were higher in Racl-shRNA[(35±5)cells],Racl-N17[(42±5)cells]and Racl-L61[(86±7)cells] groups compared with control group[(73±6)cells,P＜0.01,＜0.01 and＜0.05,resprectively].Condusion Deletoin of Racl can inhibit the invasion and migration in LDVo cells.
