Selective kinetics of HIV-1 non-nucleoside reverse transcriptase inhibitor drug resistanace-associated mutations in AIDS patients receiving highly active anti-retrovirul therapy
10.3760/cma.j.issn.1009-9158.2009.04.007
- VernacularTitle:接受抗病毒治疗的AIDS患者HIV-1非核苷类逆转录酶抑制剂类耐药基因突变的选择动力学研究
- Author:
Yu LI
;
Liyan JIAO
;
Hanping LI
;
Lin LI
;
Yongjian LIU
;
Daomin ZHUANG
;
Zuoyi BAO
;
Siyang LIU
;
Hong LI
;
Zhe WANG
;
Jingyun LI
- Publication Type:Journal Article
- Keywords:
Acquired immunodeficiency syndrome;
Reverse transcriptase inhibitors;
Drug resistance;
Mutation;
Evolution,molecular
- From:
Chinese Journal of Laboratory Medicine
2009;32(4):390-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the molecular evolutional characteristics of HIV-1 non-nucleoside reverse transcriptase inhibitor (NNRTI) drug resistance-associated mutations in AIDS patients receiving highly active antiretroviral therapy (HAART).Methods Four AIDS patients receiving HAART with good adherence within a HlV-1 drug resistance cohort from a rural region in central China were selected,who possessed susceptible virus at the beginning of treatment and gradually came to produce resistance to NNRTIs during the process of antiretroviral therapy (ART),reverse transcriptase (RT) genes from each patient's peripheral blood samples (from 3 to 30 months after withdrawal) were cloned and sequenced in succession.Results To sequenced total 855 clones and obtained the HIV-1 NNRTI drug resistance-asseciated mutations patterns of the four patients: (1)G190A often appeared with F227 L and had the tendency of accumulating P236V during the process of treatmenL (2)Y188C always presented alone and sometimes it concured with P236V.(3) YI81C frequently concured with VI79D or KIO3N and the combination varies from patient to patient.(4)K103N often combined with Y181C or M230L Conclusions The molecular evolutional characteristics of HIV-1 NNRTI drug resistance-asseciated mutations in the 4 AIDS patients are summarized.They showed different pathways on HIV-1 NNRTI drug resistance-associated mutations and those mutations detected early tend to be predominant strains.
