Effect of erythropoietin on high glucose-induced epithelial-mesenchymal transition in renal proximal tubular epithelial cells

VernacularTitle:红细胞生成素对高糖诱导下肾小管上皮细胞转分化的影响
Author: Kaiyuan ZHENG ; Hua GAN ; Xiaogang DU ; Lei LIU
Publication Type:Journal Article
Keywords: Diabetic nephropathies; Erythropoietin; Fibrosis; Epithelial-mesenchymal transition
From: Chinese Journal of Nephrology 2009;25(3):222-226
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the protective effect of erythropoietin (EPO) on human renal tubular epithelial-mesenchymal transition (EMT) induced by high glucose. MethodsCultured HK-2 cells were divided into 5 groups: normal control group, osmolarity control group, high glucose group, high glucose with EPO (5 U/ml)group and high glucose with EPO (10 U/ml)group. The mRNA expression of α-SMA, TGF-β1, Smad2, ILK of cells were measured by RT-PCR. The levels of intracellular α-SMA and TGF-β1 protein were measured by immunocytochemistry. ResultsCompared with the control groups, mRNA expression of α-SMA, TGF-β1, Smad2, ILK and protein expression of α-SMA and TGF-β1 were increased after high glucose treatment (P<0.01). Compared with the high glucose group, EPO 5 U/ml or EPO 10 U/ml remarkably down-regulated the expression of α-SMA, TGF-β1, Smad2 and ILK (P<0.01). ConclusionEPO can inhibit the progression of EMT and the up-regulation of TGF-β1, Smad2 and ILK induced by high glucose.