Protective effect of astragaloside Ⅳ on high glucose-induced podocyte adhesion and its mechanism
10.3760/cma.j.issn.1001-7097.2009.03.014
- VernacularTitle:黄芪甲苷对高糖刺激下的足细胞黏附功能的保护作用及机制
- Author:
Jianguo CHEN
;
Dingkun GUI
;
Lijun MOU
;
Yifang CHEN
;
Jianhua HUANG
;
Yi LIU
- Publication Type:Journal Article
- Keywords:
Astragaloside;
Diabetic nephropathies;
Podocytes;
Integrin alpha3betal;
High glucose;
Cell adhesion
- From:
Chinese Journal of Nephrology
2009;25(3):227-232
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of astragaloside Ⅳ(AS-Ⅳ) on glucose-induced podocyte adhesion and its possible mechanism. MethodsConditionally immortalized mouse podoeytes were treated with 10, 50, 100 mg/L AS-Ⅳ and with 100 mg/L AS-Ⅳ for 3, 6, 12, 24 h. Cell attachment was measured by fluorescence and centrifugation cell adhesion assays, respectively. Expression of α3β1 integrin mRNA and protein was examined by real-time PCR and Western blot. ResultsHigh glucose induced a significant reduction in adherent podocytes compared to normal glucose group (P<0.05). AS-Ⅳ improved high glucose-induced podocyte adhesion in a time- and dose-dependent manner. Real-time PCR and Western blot analysis revealed that high glucose-induced down-regulation of α3β1 integrin in podocytes were significantly meliorated by AS-Ⅳ (P<0.05). ConclusionAstragaloside Ⅳ improved high glucose-induced podocyte adhesion which may be mediated through α3β1 integrin up-regulation.
