The comparison of specific antibodies levels in the BALB/c mice immunized with the pcDNA3.1 recombinant plasmids of E gene partial sequence from different Dengue type 2 virus strains

VernacularTitle:两株登革2型病毒E基因重组质粒免疫BALB/c小鼠产生特异性抗体水平的比较
Author: Jiao WANG ; Li ZUO ; Cong WANG ; Xuezheng LI
Publication Type:Journal Article
Keywords: Dengue type 2 virus; Envelope gene; Recombinant plasmid; BALB/c mice; Specific antibody
From: Chinese Journal of Microbiology and Immunology 2009;29(3):229-233
CountryChina
Language:Chinese
Abstract: Objective To compare humoral immune response of BALB/c mice immunized by recombinant plasmids poDNA3.1-BE and pcDNA3.1-NE. Methods The BALB/c mice were immunized by recombinant plasmids pcDNA3.1-BE, pcDNA3.1-NE with adjuvant. Each animal received a primary inoculation and two boosts at 2-week intervals. Then the blood samples of BALB/c mice were collected from different experiment groups at day 14, 28, 42, 70 and 98, respectively after first immunization. The specific IgM/IgG antibodies for E protein in serum were confirmed by indirect ELISA. And then the activities of the specific neutralizing antibody were determined by cytopathic effect inhibition (CPEI). Results The levels of specific IgM/IgG antibodies and neutralizing antibody activities were increased in PODNA3.1-BE enhance immunization group than that of other groups. The result had been observed longer duration of antibody level in pcDNA3.,-BE enhance immunization group. Conclusion Humoral immune response were significant differences between recombinant plasmid pcDNA3.1-BE and PcDNA3.1-NE immunized mice groups.