Development and clinical application of fluorescent real-time RT-PCR to hand, foot and mouth disease
10.3760/cma.j.issn.0254-5101.2009.03.023
- VernacularTitle:手足口病病原体实时荧光RT-PCR反应体系的建立与临床应用
- Author:
Yongle ZHANG
;
Kenu PAN
;
Dai XU
;
Weifeng LIANG
;
Guoqiang LOU
- Publication Type:Journal Article
- Keywords:
Hand,foot and mouth disease;
Enterovirus;
Coxsackie virus A16;
Real-time RT-PCR
- From:
Chinese Journal of Microbiology and Immunology
2009;29(3):276-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid, accurate, specific method to detect causative agent of hand, foot and mouth disease (HFMD). Methods Specific primers and probe were designed based on highly conserved VP1 region of enterovirus 71, coxsackie virus A16 and enterovirus. The sensitivity and specificity of the real-time RT-PCR was evaluated with 35 stool samples collected from pediatric patients with suspected HFMD and 20 clinical samples from health pediatric patients. Results Out of 35 clinical samples from suspected HFMD, 35 samples were identified as positive for enterovirus, 25 clinical samples were identified as positive for enterovirus 71, 8 clinical samples were identified as positive for coxsackie virus A16, among which 3 clinical samples were identified as positive for enterovirus 71 and coxsackie virus A16. The clinical diagnostic accordance rate is 85.71%. Out of 20 clinical samples from normal pediatric patients, 5 clinical samples were identified as positive for enterovirus, 20 clinical samples were negative for enterovirns 71 and coxsackie virus AI6. Conclusion Our results indicate real-time RT-PCR offers a rapid, sensitive, specific and cheap method to detect pathogen of HFMD from clinical specimens.
