The research about Candida albicans biofilms eliminated by miconazole combined with drug efflux pump inhibitors
10.3760/cma.j.issn.0254-5101.2009.02.004
- VernacularTitle:药物流出泵抑制剂联合咪康唑清除白假丝酵母菌生物被膜的研究
- Author:
Qingguo QI
;
Wenxia WANG
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Biofilm;
Drug resistance;
Drug efflux pump
- From:
Chinese Journal of Microbiology and Immunology
2009;29(2):113-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of killing the biofilm persisters by miconazole combined with two compound which can inhibit the drug efflux of C. albicans. Methods Lab reference strains of C. albicans YEM30(CAF2-1) formed the mature biofilm in the 96 well plates, and then treated with miconazole combined with Enniatin B (CDR1 inhibitor) and Milbemycins ot25 (CDRI/CDR2 inhibitor) respectively. After incubated for 48 hours by CFU counting on the YPD plates, the analysis of persisters with SAS8.0 software package for q test. Results Miconazole combined with drug efflux inhibitor can kill more persisters than miconazole alone(P <0.001), and combining with Enniatin B have a better results in eliminating the biofilm persisters than with Milbemycins α25. Conclusion Antifungal drugs combined with drug efflux pump inhibitors can kill more strains which can tolerate very high concentration of antifungal drugs. And searching the potential drug efflux pump inhibitors may be a new way for eliminating the persisters in biofilm, and consequently controlling the chronic recurrent fungal infectious diseases.
