Establishment of mouse model for human metapneumovirus infection
10.3760/cma.j.issn.0254-5101.2009.04.006
- VernacularTitle:小鼠人偏肺病毒感染模型的建立
- Author:
Ying DOU
;
Yao ZHAO
;
Zhiyong ZHANG
;
Xiaodong ZHAO
- Publication Type:Journal Article
- Keywords:
Human metapneumovirus;
BALB/c mice;
Animal model
- From:
Chinese Journal of Microbiology and Immunology
2009;29(4):312-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model for human metapneumovirus (hMPV) infec-tion, and to explore the course of viral replication and pathology and lay ground for vaccine development. Methods BALB/c mice were infected intranasally with hMPV, and sacrificed on day 1,3, 5, 7, 9 and 16 post inoculation. Lungs were used for viral isolation, viral titration by an improved plaque assays, pulmonary histopathology and detection of hMPV mRNA expression by RT-PCR. Results Live viruses were successful-ly isolated from the lungs of infected mice, hMPV replicate well in the lungs and viral titers peaked on the 5th day post inoculation (5.16 ± 1.09)×105 PFU/g, remaining to be detectable on the 9th day post inocu- lation (2.79 ± 1.22)×102 PFU/g. Genomic RNA of hMPV was able to be detected on the 16th day post in-oculation by RT-PCR targeting F gene. Histopathology of lungs was characterized by interstitial pneumonia in particular during 3 ～ 7 days post inoculation. Conclusion hMPV infected BALB/c mice model was success-fully established, hMPV infected mice showed similar patterns of viral replication and pulmonary histopathol-ogy as in infected humans.
