Epidemiological investigation of Borna disease virus infection in horses and donkeys in Yili, Xinjiang
10.3760/cma.j.issn.0254-5101.2009.04.008
- VernacularTitle:新疆伊犁地区马和驴博尔纳病病毒自然感染的调查
- Author:
Yiagying ZHANG
;
Qunling ZHAN
;
Mingming XU
;
Jianping YU
;
Zhilei ZENG
;
Hong ZHA
;
Yanxi LIU
;
Xiao CHEN
;
Dan PENG
;
Dan ZHU
;
Yongbo HU
;
Kang HUO
;
Peng XIE
- Publication Type:Journal Article
- Keywords:
Borua disease virus;
Fluorescence quantitative nested RT-PCR;
TaqMan probe;
Yilihorses;
Yili donkeys
- From:
Chinese Journal of Microbiology and Immunology
2009;29(4):321-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epidemiology of BDV infection in Yili horses and Yili donkeys and to analyze phylogenetic source of BDV in Yili area, Xinjiang. Methods We established fluo- rescence quantitative nested RT-PCR to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) of 518 Yili horses and 206 Yili donkeys in Yili area, Xinjiang. Positive products were validated by detecting BDV p40 segment and plasmid to preclude the contamination, and were sequenced to analyze the homology of gene sequence, amino acid sequence and phylogenetic tree. Results The positive rates of BDV infection in PBMCs of 518 Yili horses and 206 Yili donkeys were 0.97% and 1.94%, respectively. The results of BDV p40 segment verification were positive in all of the samples of BDV p24 positive. All the samples tested were not contaminated by plasmid. There was a homology of the gene sequence of positive PCR samples with strain He/80. And the gene sequence revealed more than 93% identical to H1766 and strain V. Conclusion Our study suggested BDV natural infection in Yili horses and Yili donkeys. The en- demic BDV had a high degree of identity to strain He/80.
