Up-regulate of the proportion of CD4+CD25+ regulatory T cells by lymphoma cell EL-4 supernatants

VernacularTitle:淋巴瘤细胞EL-4培养上清液对CD4+CD25+调节性T细胞比例的调节作用
Author: Yusheng CHENG ; Fan ZHANG ; Guiqin WANG
Publication Type:Journal Article
Keywords: Cell line,tumor; T-lymphocytes,regulatory; Foxp3 mRNA
From: Cancer Research and Clinic 2009;21(4):239-241
CountryChina
Language:Chinese
Abstract: Objective To explore if the tumor cell can up-regulate the proliferation of CD4+CD25+ Treg cells. Methods Lymphoma cell EL-4 supernatant was combined with the normal mise spleen lymphocyte. After cultured 72 hours, CD4+CD25+ subset were analyzed by flow cytometry and the gene expression level of the specific marker Foxp3 were tested by RT-PCR. The experiment repeated three times. Results The supematant derived from EL-4 can increase the proportion of CD4+CD25+ Treg cells in normal mouse spleen lymphocytes than that of the control group. Moreover, when added the CD3 McAb with EL-4 supematant together, the number of CD4+CD25+ Treg cells increased. The level of corresponding Foxp3 mRNA also increased. Conclusion These data suggested that in the supernatant may occur immune regulatory factors which up-regulated the number of CD4+CD25+ Treg cells and indicated that the tumorigenesis can facilitate proliferation of CD4+CD25+ Treg cells.