The proliferative effect on silencing of endogenetic mdr1 gene expression by RNA interference in ovarian cancer resistant strain SKOV3/TAXOL

VernacularTitle:RNAi沉默卵巢癌耐药细胞株SKOV3/TAXOL内源mdr1基因对细胞增生的影响
Author: Jiliang FENG ; Hongmei WANG ; Huanling YAO ; Chuanxia MA ; Xiuping DU
Publication Type:Journal Article
Keywords: Ovarian neoplasms; RNA interfering; Cell line,tumor; Genes,mdr1; Hyperplasia
From: Cancer Research and Clinic 2009;21(4):229-231
CountryChina
Language:Chinese
Abstract: Objective To construct the short hairpin RNA recombinant plasmids targeting mdr1 gene which expresses highly in ovarian cancer resistance strain SKOV3/TAXOL to silence endogenefic mdr1 gene expression and investigate the role of mdr1 gene in the development of resistant ovarian cancer. Methods The pGPU6/GFP/Neo-mdr1 were constructed by gene clone technology. The influence on proliferation and apoptosis were investigated by CCK-8 in SKOV3/TAXOL after transfected pGPU6/GFP/Neo-mdr1. Results The expression against mdr1 proteins were inhibited by pGPU6/GFP/Neo-mdr1. The cell proliferation were inhibited after transfected pGPU6/GFP/Neo-mdr1 by CCK-8. The apoptosis were observed in DAB experiments and the apoptosis rate increased. Conclusion mdr1 plays an important role in proliferation of resistant ovarian cancer and the short hairpin RNA of mdr1 can efficiently suppress mdr1 expression and enhance the apoptosis in SKOV3/ATAXOL.