The effects of antibiotics on gene expression of CD14 and pro-inflammatory/anti-inflammatory cytokines in liver of Vibrio vulnificus septic rats
10.3760/cma.j.issn.0254-5101.2009.03.017
- VernacularTitle:创伤弧菌脓毒症大鼠肝组织CD14和促/抗炎细胞因子基因表达及抗生素干预研究
- Author:
Huan LIANG
;
Zhongqiu LU
;
Qiaomeng QIU
;
Jingrong LI
;
Guangliang HONG
;
Mengfang LI
;
Tieli ZHOU
- Publication Type:Journal Article
- Keywords:
Vibrio vulnificus;
Sepsis;
Lipopolysaccharide receptor CD14;
Tumor necrosis factor-α (TNF-α);
Interleukiu-10 (IL-10)
- From:
Chinese Journal of Microbiology and Immunology
2009;29(3):253-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential role and changes of CD14, TNF-ct and IL-10 in liver in Vibrio vulnificus septic rats, and detect the intervention effects of eefoperazone sodium combined with levofloxacin. Methods To make Vibrio vulnificus sepsis model (VV group) and drug intervention model (AA group) in rats, the expression of CD14, TNF-α, IL-10 in liver were detected by RT-PCR. Results Compared with normal control (NC) group,CD14 mRNA and TNF-α mRNA expression increased markedly at 2, 6, 9, 12, 16 h in VV group (P<0.05), IL-10 mRNA raised greatly at 9, 12, 16 in VV group (P<0.05). CD14 mRNA expression was also rised in AA group at 9 h(P<0.05). TNF-α mRNA at 9, 12 h and IL-10 mRNA at 9, 12, 16 h in the AA group increased (P<0.05). Compared with VV groups, CD14 mRNA expression diminished greatly at 9, 12, 16 h in AA group (P <0.05), TNF-α mRNA and IL-10 mRNA diminished in the AA group at 16 h(P<0.05). Conclusion The treatment with cefoperazone sodium and levofloxacin may reduce expression of CD14, TNF-α and IL-10 in liver of rats with VV sepsis, it may inhibit the level of pro-inflammatory/anti-inflammatory cytokines, thereby regulating the balance of the inflammatory response in VV sepsis.
