Interleukin-1 beta gene polymorphisms and variation in whole genomic expression profiles of chronic atrophic gastritis
10.3760/cma.j.issn.0254-1432.2009.05.011
- VernacularTitle:白细胞介素-1β基因多态性与慢性萎缩性胃炎基因表达谱的变化
- Author:
Shaoying WANG
;
Xiaoying SHEN
;
Caiyun WU
;
Feng PAN
;
Yuanyuan SHEN
;
Haihui SHENG
;
Ximei CHEN
;
Hengjun GAO
- Publication Type:Journal Article
- Keywords:
Chronic atrophy gastritis;
Interleukin-1 beta;
Single nucleotide polymorphism Gene expression profile
- From:
Chinese Journal of Digestion
2009;29(5):326-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the whole genomic expression profiles of chronic atrophic gastritis with interleukin(IL)-1β-31CC/-511TT genotype as measured by oligonucleotide microarray technique.Methods Genomic RNA was extracted from gastric biopsies of 12 patients with chronic atrophic gastritis(6 with IL-1β-31CC/-511TT and 6 with IL-1β-31TT/-511CC).The genomic profiles of IL-1β gene polymorphisms 31CC/-511TT and 31TT/-511CC were compared and tested for differential expressed genes associated with 31CC/-511TT using Agilent human whole genomic oligonucleotide microarrays.The results were further analyzed in terms of gene ontology(GO).Results There were 200 differentially expressed genes associated with IL-1β-31CC/-511TT,159 of which were up-regulated and 41 were down-regulated.These genes mainly involved in macromolecule metabolic process,post-translational protein modification,ubiquitin cycle,and protein kinase cascade.Five genes had biological activities,one of which was down-regulated gene(PCSK5)and 4 were upregulated genes(PRKCA,NPLOC4,TRIB3 and MAPKAPK3).Conclusions The chronic atrophic gastritis with IL-1β-31CC/-511TT genotype has molecular phenotypes which is associated with malignance and inflammation.These individuals are needed more intensive preemptive treatment and dynamic surveillance.
