The isolation of Corynebacterium tuberculostearicum from prostatic fluid
10.3760/cma.j.issn.0254-5101.2009.04.002
- VernacularTitle:从前列腺液中分离到结核硬脂酸棒状杆菌
- Author:
Qian YUE
;
Zhenwen QIAN
;
Yuanyuan YANG
;
Shumei ZHAO
;
Ying HUANG
;
Guangxiang JIN
;
Ying GONG
;
Naixin ZHAO
- Publication Type:Journal Article
- Keywords:
Prostatitis;
Corynebacterium tuberculostearicum;
Taxonomic identification;
Phylogeny
- From:
Chinese Journal of Microbiology and Immunology
2009;29(4):294-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To carry out a taxonomic identification of a strain of claviform bacteria iso-lated from prostatic fluid of a patient who suffered from chronic prostatitis, and to approach its phylogenic and biologic position. Methods We undertaked an initial identification by phenotypic characters such as morphologecal, physiological and biochemical characteristics to ascertain its phylogeny by chemical composi-tion analysis of cell wall and 16S rRNA gene sequencing and alignment. Results A club-shaped gram posi-tive rod bacillus was isolated in pure culture state. Its biochemical reactions were not active. The diamino-acid of cell wall was meso-diaminopimelic acid (meso-DAP) and it had wall chemotype Ⅳ ( contained arabi-nose, galactose and maltose ). Sequence searches of the GenBank database revealed that this strain had a highest level of 16S rDNA sequence similarity (99.4%) to C. tuberculostearicurn strain ATCC35692 with only 8 nucleotides difference. Conclusion On the basis of phenotypic and phylngenetie analysis, it is rea-sonable to assign this strain to the species C. tuberculostearicum, and this is the first isolation of C. tubercu-lostearicum from prostatic fluid home and abroad.
