Insulin has no effect on vascular endothelial growth factor expression in the retina of streptozotocin-induced diabetic rats
10.3760/cma.j.issn.1005-1015.2009.03.012
- VernacularTitle:胰岛素对链脲佐菌素诱导的糖尿病大鼠视网膜血管内皮生长因子表达的影响
- Author:
Haixiang WU
;
Xin XIA
;
Qing GU
;
Xun XU
- Publication Type:Journal Article
- Keywords:
Insulin/ physiology,Diabetic retinopathy/ etiology;
Vascular endothelial growth factors / analysis;
Disease models,animal
- From:
Chinese Journal of Ocular Fundus Diseases
2009;25(3):202-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate if insulin can affect the expression of vascular endothelial growth factor (VEGF) in the retina of streptozotocin-induced diabetic rats. Methods A total of 60 male Sprague-Dawley rats were randomly divided into sodium citrate buffer control group (CIT-CON, n= 30) and STZ-induced diabetic group (STZ-DM, n=30). At the 16th week, 24 rats from CIT-CON group at random were randomly divided to group A (sodium citrate buffer control group, n = 12) and group B (sodium citrate buffer plus insulin group, n= 12). The remaining 6 rats from as CIT-CON group served as negative control. At the same time, 24 rats from STZ-DM group at random were randomly divided to group C (STZ-induced diabetic group, n= 12) and group D (STZ-induced diabetic plus insulin group, n= 12). The remaining 6 rats from STZ-DM group also served as negative control. 4 IU of insulin was injected subcutaneously to rats of group B and D. Immunohistochemistry, Western blot and Real-time polymerase chain reaction (RT-PCR) were used to measure the expression level of VEGF protein and mRNA respectively. RESULTS Insulin significantly increased the VEGF mRNA (7.71 ± 0.25 vs 5.36 ±0. 37, t test P< 0. 05) and protein expression (0. 4925 ± 0. 0122 vs 0. 4272 ± 0. 0110, t test P< 0. 05) in the retina of CIT-CON rats.However, in retina of STZ-DM rats, insulin had no effect on VEGF mRNA (8. 92±0. 27 vs 9. 05±0. 28, t test, P>0. 05) and protein expression (0. 5152±0. 0109 vs 0. 5099±0. 0100, t test P>0.05). Conclusions Insulin had no effect on VEGF expression in the retina of STZ-DM rats.
