Study of resistance to carbapenems of Pseudomonas aeruginosa in 5 Beijing hospitals

VernacularTitle:北京地区五家医院获得性铜绿假单胞菌对碳青霉烯类抗菌药物耐药机制研究
Author: Chunxia YANG ; Qingtao WANG ; Fang LI ; Binbin LI ; Xiaoling DU ; Bai XIAO
Publication Type:Journal Article
Keywords: Pseudomonas aeruginosa; Imipenem-resistant; Metallo-β-lactamase; OprD,2
From: Chinese Journal of Laboratory Medicine 2009;32(5):538-542
CountryChina
Language:Chinese
Abstract: Objective To investigate the molecular mechanism of resistance to carbapenems in Pseudomonas aeruginasa from 5 teaching hospitals in Beijing. Methods A total of 213 non-duplicate imipenem-resistant Pseudomonas aernginosa (IRPA) isolates were collected from 5 hospitals in Beijing from June 2004 to December 2005. The minimal inhibitory concentrations (MICs) of meropenem, imipenem and others antibiotics were determined by agar dilution method. Disk diffusion test was used for screening metalloenzymes. The bla IMP,bla VIM and OprD2 genes were amplified by PCR and only sequenced. Results Out of 213 isolates, OprD2 loss was detected in 84 isolates and IMP-1 enzyme was detected in 6 isolates simultaneously. Thirteen IRPAs only produced IMP-1 and 2 isolates only produced VIM-2. Conclusion OprD2 loss and metallo-β-lactamuse production are the parts of the mechanisms of resistance to carbapenems in Pseudomonas aeruginosa in Beijing.