Detection of Escherichia coli O157:H7 by multiplex PCR-DHPLC
10.3760/cma.j.issn.0254-5101.2009.04.019
- VernacularTitle:多重PCR-DHPLC技术检测肠出血性大肠杆菌O157:H7基因型的方法学研究
- Author:
Junyi XU
;
Jijuan CAO
;
Qiuyue ZHENG
;
Qiuyan WANG
;
Shuyan LIU
;
Dan JIANG
- Publication Type:Journal Article
- Keywords:
E.coli O157: H7;
Multiplex PCR;
Denaturing high performance liquid chromatogra-phy
- From:
Chinese Journal of Microbiology and Immunology
2009;29(4):366-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the PCR-denaturing high performance liquid chromatography (DHPLC) for detection of E. Coli O157: H7. Methods The virulence genes of Shiga-like toxin(SLT) and rfbE were specifically amplified by 2 sets of primers. The target gene fragments of the PCR assay were 224 bp and 499 bp, respectively. Results Analysis of 37 strains demonstrated that this PCR system was specific. The detection limit of the PCR was 4 CFU/ml. Conclusion These results indicated that the multiplex PCR-DHPLC assay can be used for specific and sensitive detection of E. Coli O157:H7.
