Effect of rhDCN on the expression of HLA molecules on HepG2 cell lines
10.3760/cma.j.issn.1006-9801.2009.05.004
- VernacularTitle:重组hDCN对肝癌细胞株HepG2表面HLA分子表达的影响
- Author:
Yonhong WANG
;
Guiqin WANG
;
Jingjing WANG
;
Fang HAO
;
Wanfang YANG
;
Ying XUE
- Publication Type:Journal Article
- Keywords:
Proteoglycans;
HepG2;
Genes,MHC class Ⅰ;
Genes,MHC class Ⅱ(2007011107)
- From:
Cancer Research and Clinic
2009;21(5):298-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the regulatory effect of recombinant human DCN on the expression of HLA class Ⅰ, Ⅱ molecule on hepatoma carcinoma cell HepG2 and investigate the relatively immune mechanism of human DCN on enhancing anti-tumor effect. Methods After transfected HepG2 cells with pcDNA3.1 (+)-DCN by liposome transfection, the expression of HLA class Ⅰ , Ⅱ molecule and the apoptotic indexes were analyzed by the flow cytometer. Results The expression of HLA class Ⅰ , Ⅱ molecule on the HepG2 cells transfected with pcDNA3.1 (+)-DCN was up-regulated with the apoptosis indexes being significantly higher than that of other control groups. Conclusion The human DCN can induce HepG2 apoptosis and up-regulate the expressions of HLA class Ⅰ , Ⅱ molecule which may contribute to its antitumor effect.
