The analysis of 5 HIV-1 gpl20 sequences from different clades and expression of their corresponding proteins in vitro
10.3760/cma.j.issn.0254-5101.2009.05.010
- VernacularTitle:5株不同亚型HIV-1毒株gp120序列特征分析及其蛋白的表达
- Author:
Zheng WANG
;
Jingyun LI
;
Zuoyi BAO
;
Hanping LI
;
Daomin ZHUANG
;
Siyang LIU
;
Lin LI
- Publication Type:Journal Article
- Keywords:
HIV-1;
gp120;
Glycoprotein
- From:
Chinese Journal of Microbiology and Immunology
2009;29(5):424-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize 5 gpl20 sequences from mainly circulating clades in China and expression of their gp120 glycoproteins. Methods gp120 genes were amplified from the PBMCs of 5 HIV-1 infected individuals in different provinces using nest PCR and their DNA sequences were determined. Sequence characteristics were analyzed and gp120 genes were sub-cloned into the mammalian expression vec-tor to produce gp120 glycoproteins. Results Sequence characteristics indicated these sequences belong to the clade Thai-B, CRF_BC and CRF_AE, respectively. There were some conservative N-linked glycosyla-tion sites and primary Furin protease cleavage motifs in the same positions within gp120 amino acid se-quences although these gp120 sequences were categorized into different clades. In comparison with referen-tial strains, amino acids deletions were found in the V1, V2, V4, V5 regions except for the V3 loop; above all, V3 tip motifs of Thai-B exhibited the more polymorphic forms than those of CRF_BC and CRF_AE. These 5 gp120 sequences were cloned into the eukaryotic expression vector and gpl20 glycoproteins were produced successfully. Conclusion Hyper-variable nature of Env should be considered while designing HIV-1 vaccine or test reagent, and gpl20 expression in vitro is helpful to further research on the Env patho-genesis and vaccine development against the mainly circulating HIV-1 isolates in China.
