Study on the relationship between erythromycin sensitivity and ermB gene in Ureaplasma urealyticum

Tinglu YE; Chun LU; Rongbiao LU; Han MA; Chuanjie Chen; Wei Lai; Guoxing Zhu; Jian Chen

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Study on the relationship between erythromycin sensitivity and ermB gene in Ureaplasma urealyticum

VernacularTitle:解脲脲原体对红霉素体外耐药性与ermB基因相关性研究
Author: Tinglu YE ; Chun LU ; Rongbiao LU ; Han MA ; Chuanjie CHEN ; Wei LAI ; Guoxing ZHU ; Jian CHEN
Publication Type:Journal Article
Keywords: Ureaplasma urealyticum; Erythromyein; Drug sensitivity test; ermB gene
From: Chinese Journal of Microbiology and Immunology 2009;29(5):472-475
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between erythromyein sensitivity and ermB gene in 143 Ureaplasma urealyticum (Uu) clinical isolates. Methods We detected the minimum inhabit concen-trations (MICs) of Uu to erythromycin by broth dilution method and MIC≥8 μg/ml was used as standard concentration of resistance to erythromycin. Polymerase chain reaction was used to detect the ermB gene and biotype Uu with primers based on multi-band antigen gene. Results The MICs, MIC50 MIC90 of Uu to erythromycin were ≤0. 125 μg/ml to ≥128 μg/ml, 16 μg/ml, and ≥128 μg/ml, respectively, with a high resistance rate of 64.38%. ermB gene, which was mainly detected in Uu with MIC≥8 μg/ml, was positively detected in 40 out of 143 Uu strains (27.97%). No significant differences of the resistance to erythromycin and positive rate of ermB gene were found between the two biovars in the study . Conclusion ermB gene may probably be one of the important genes conferring resistance to erythromycin in Uu. Further studies are needed to discover the difference of resistance and mechanism of erythromycin between the two bi-ovars.