The relationship between TNF-αγ, IFN-γ and intestinal mucosal permeability in DSS-induced colitis and its effect by balsalazide
10.3760/cma.j.issn.0254-5101.2009.05.002
- VernacularTitle:巴柳氮对结肠炎小鼠肠TNF-α、IFN-γ和黏膜通透性影响的研究
- Author:
Xiaochang LIU
;
Qiao MEI
;
Jianming XU
;
Juan JIN
- Publication Type:Journal Article
- Keywords:
Colitis;
DSS;
Balsalazine;
Cytokine;
Intestinal mucosal permeability
- From:Chinese Journal of Microbiology and Immunology
2009;29(5):395-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between TNF-α, IFN-γand intestinal muco-sal permeability in a mouse colitis model and its inhibiting effect by balsalazide. Methods Forty-five C57BL/6J mice were divided randomly into five groups. Normal group was only fed with distilled water, DSS group and balsalazide groups at doses of 42, 141,423 mg/kg were both fed with 5% DSS. Balsalazide was given by intragastric administration. At the end of the experiment, colon tissue was collected for assessment of histological index(HI) and the MPO activity. Small intestinal mucosa was collected for assessment of the content of TNF-α and IFN-γ,transmission electron microscope(TEM), and detection of permeability by Ev-arts blue method. Results Compared with normal group, DSS group mice all manifested severe weight loss associated with hematocbezia and diarrhea, HI score, and the colon MPO activity and the content of TNF-α and IFN-γ were increased significantly. Intestinal mucosa showed a thinning of microvillous carpet, with de-curtated and broaden junctional complex and enlarged intercollutar space under TEM observations. The amount of Evans blue permeated into intestinal wall was obvious. Compared with DSS group, the HI score, the MPO activity and the content of TNF-α and IFN-γ were decreased by balsalazide. The amount of Evans blue permeated into intestinal wall was less. Ileal microvillous carpet was ameliorated dose dependently by balsalazide. Conclusion In DSS-induced colitis model, the change of the content of the TNF-α and IFN-γ, was accordance with the increase of intestinal mucosal permeability while balsalazide can significantly amelio-rate intestinal mucosal permeability by its anti-colitis effect.
