Comparation of magnetic activated cell sorting and chemotherapy agent DDP in enriching cancer stem cell of laryngeal carcinoma cell line Hep-2

VernacularTitle:免疫磁珠分选与顺铂富集喉癌Hep-2细胞系中肿瘤干细胞的比较
Author: Pingping LIU ; Xiaoming LI ; Xiuying LU
Publication Type:Journal Article
Keywords: Cancer stem cell (CSC); Enrich; Magnetic activated cell sorting (MACS); Chemotherapy agents; CD133
From: Cancer Research and Clinic 2009;21(5):304-307
CountryChina
Language:Chinese
Abstract: Objective To compare effect of chemotherapy agent DDP to MACS in sorting cancer stemcells (CSC) of laryngeal carcinoma cell line Hep-2. Methods CD133 magnetic beads were applied to sort Hep-2 cells. Different dosages of DDP were used to treat Hep-2 cells for 48 hours. Enrichment rate of CD133+ cells by MACS and after DDP treatment was detected by Flow Cytometer (FCM). Morphologic change was observed under inverse-phase microscope. Results FCM showed that the sorting rate of CD133+ cells through MACS was 64.33 %, while after DDP treatment for 48 hours, the rate of CD133+ cells was enriched significantly in each dosage of DDP, with the maximal rate was 50.7 %, in the dosage of 4 μg/ml. There was a significantly difference between MACS and each of DDP group (P <0.01). Cells treated with DDP were abnormal in morphology. Conclusion MACS and DDP sorting has respective advantages in enriching CSC in Hep-2 cell lines.