Expression changes of intermedin and calcitonin receptor-like receptor in the kidney of rats after renal ischemia reperfusion injury
10.3760/cma.j.issn.1001-7097.2009.05.016
- VernacularTitle:大鼠肾脏缺血再灌注损伤中垂体中叶素及降钙素受体样受体的表达
- Author:
Guihua YU
;
Rongshan LI
;
Xi QIAO
;
Yun ZHOU
;
Min KOU
;
Chen WANG
;
Bo BAI
;
Shan SHAO
- Publication Type:Journal Article
- Keywords:
Adrenomedullin;
Receptors,calcitonin;
Reperfusion injury;
Kidneytubular necrosis,acute
- From:
Chinese Journal of Nephrology
2009;25(5):387-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of intermedin /adrenomeduliin 2 (IMD/AM2) and its receptor calcitonin receptor-like receptor (CRLR) in the kidney of rats after renal ischemia reperfusion injury(IRI). Methods Male Wistar rats were divided randomly into two groups: sham group and operation group. Renal IRI model was induced by clamping both renal arteries. Blood and kidney were harversted at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h after reperfusion, respectively. Renal histological changes were semi-quantitated. Expressions of IMD and CRLR in the kidney were detected by Western blot, and the content of IMD in serum was measured by radioimmunity at 12 h, 48 h, 72 h after repeffusion. Results Kidneys of renal IRI model rats displayed significant pathologic changes, and the changes were much severer at 48 h after reperfusion. The expressions of IMD and CRLR in kidney were significantly up-regnlated at 12 h, 48 h, 72 h after renal IRI (P<0.01). The level of IMD in serum increased at 12 h, 48 h, 72 h after renal IRI (P<0.05). Conclusion The expressions of IMD and its receptor are up-regulated in the kidney after renal IRI, which may participate in the pathophysiological changes induced by renal IRI.
