The effect of electro-acupuncture on bladder detrusor apoptosis in rats with urinary retention after spinal cord injury
10.3760/cma.j.issn.0254-1424.2009.06.007
- VernacularTitle:电针不同穴位对脊髓损伤后尿潴留大鼠逼尿肌细胞凋亡的影响
- Author:
Junhua WANG
;
Bangguo CHEN
;
Jing YIN
;
Weigeng ZOU
;
Xiaojuan LUO
;
Xiaoming ZHANG
- Publication Type:Journal Article
- Keywords:
Electro-acupuncture;
Effect of point;
Guanyuan acupoint;
Shuidao acupoint;
Detrusor;
Apoptosis
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2009;31(6):380-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of electro-acupuncture(EA)on bladder detrusor apoptosis in rats with urinary retention after spinal cord injury(SCI),compare the effectiveness of different acupoints and explore possible mechanisms.Methods One hundred female adult Sprague-Dawley rats were randomly divided into 5 groups:a normal group,a sham operation group,a model control group,a Guanyuan acupoint(RN4)EA group and a Shuidao acupoint(ST28)EA group,with 20 rats in each group.A model of urinary retention after SCI was established using the weight drop method.The EA groups were given EA once daily for 10 d.The other groups had no treatment.After 10 d of EA,an electron microscope was used to observe the bladder detrusor ultrastructure,and the terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) method and immunohistochemistry were used to observe any apoptosis in the detrusor smooth muscle cells and the expression of apoptosis-related genes bcl-2 and bax.Results After EA treatment,expansion of the rough endoplasmic reticulum was reduced significantly,and the number of edemic mitochondria was reduced compared with the model control group.In the normal and sham operation groups,little apoptosis was observed,and there was no significant difference between the two groups.Apoptosis indices(AIs)were higher in the model control group than in the normal group.The average AI in the Guanyuan EA group was lower than that in the Shuidao group.In the Guanyuan group,bax protein expression was less than that in the Shuidao group and the model group.In the Guanyuan group,bcl-2 protein expression was not significantly different from that in the Shuidao group and the model control group.Conclusion EA at the Guanyuan or Shuidao aeupoint can restrain bladder detrusor apoptosis in rats with urinary retention after SCI.EA may improve the ultrastructure of the detrosor urinae, reduce the expression of bax, change the ratio of bcl-2 to bax, and thus decrease apoptosis.The effect of EA at the Guanyuan acupoint is superior to that at the Shuidao acupoint.
