Sequence variability of human cytomegalovirus UL143 open reading frame gene in low-passage clinical isolates

VernacularTitle:人巨细胞病毒UL143基因在临床低传代分离株中的多态性研究
Author: Rong HE ; Qiang RUAN ; Ying QI ; Yanping MA ; Yaohua JI
Publication Type:Journal Article
Keywords: Human cytomegalovirus; UL143 gene; Polymorphism
From: Chinese Journal of Microbiology and Immunology 2009;29(6):491-494
CountryChina
Language:Chinese
Abstract: Objective To explore the relationship between ULI43 sequence variability and clini-cal disease. Methods UL143 from samples obtained from suspected congenitally human cytomegalovirus (HCMV) infected symptomatic infants were PCR amplified and sequenced. Results There were not too much sequence variability of UL143 compared with Toledo. But no one was completely identical to Toledo, and all UL143 ORFs were shorter than Toledo for frame-shift. Conclusion HCMV-UL143 existed in moat of low passage isolates and sequences were variable. No obvious linkage was observed between UL143 poly-morphisms and outcome of suspected congenital HCMV infection.