Experimental study on relationship between Smad4 and angiogenesis related factors in human gastric cancer cell line

VernacularTitle:Smad4基因对人类胃癌细胞株血管生成相关因子影响的研究
Author: Shidan CHENG ; Feng GAO ; Xiaojin WANG ; Minmin QIAO ; Yunlin WU
Publication Type:Journal Article
Keywords: Stomach neoplasms; Cell line,tumor; Vascular endothelial growth factor; Thromhospondin 1; Smad4
From: Chinese Journal of Digestion 2009;29(6):385-388
CountryChina
Language:Chinese
Abstract: Objective To study the effect of Smad4 gene on angiogenesis related factors in human gastric cancer cell line.Methods Recombinant eukaryotie expressing plasmid pcDNA3.1 (-)-Smad4 containing Smad4 gene and empty vector pcDNA3.1 (-) were introduced into human gastric cancer cell line MKN28 using lipofectam and selected by G418,respectively.Two cell lines were obtained as follows:Smad4+-MKN28 cell line which was MKN28 transfected with a stable hybrid containing Smad4 gene and Smad4--MKN28 cell line with empty plasmid as control.The transcription and expression of VEGF and TSP1 were investigated by RT-PCR and Western blot.Results The mRNA expression of TSP1 in Smad4+-MKN28 cells was higher (P＜0.05) than that in control cells,while VEGF was lower(P＜0.05).Western blot showed the consistent results as measurement by RT-PCR.Conclusion Smad4 restoration in gastric cancer cells reduced angiogenesis rates through down-regulation of angiogenesis activitor and up-regulation of angiogenesis inhibitor.