Expression of polysaccharides biosynthetic genes in biofilm development in Pseudomonas aeruginosa
10.3760/cma.j.issn.0254-5101.2009.06.008
- VernacularTitle:铜绿假单胞菌生物被膜形成过程中多糖合成相关基因表达的研究
- Author:
Liyan LI
;
Dianjun WEI
;
Kun MEN
;
Dawei WU
;
Jinying CHEN
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Biofilm;
Polysaccharides
- From:
Chinese Journal of Microbiology and Immunology
2009;29(6):513-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expression of polysaccharides biosynthetic genes in biofilm formation in Pseudomonas aeruginosa PAO1. Methods Real-time RT-PCR was used to quantify mRNA to analyze their mRNA level during planktonic growth and biofilm cells. Results Analysis of the relative ex-pression levels indicated that the level of transcripts of planktonic pslA, algD, pelA was significantly lower than that of biofilms sessile growth in all other form bacteria. The levels of transcripts of pslA, algD, pelA were the highest at the first day of biofilms development. Conclusion From the observations in the present study, transcripts of the pslA, algD, pelA involved biofilm development.
