Effect of trehalose on expression of E-cadherin in cryopreserved human skin
- VernacularTitle:海藻糖对低温保存皮肤中E钙黏素表达的影响
- Author:
Hongmei LIU
;
Hong CAI
;
Ling LIU
;
Taichao DUI
- Publication Type:Journal Article
- Keywords:
Skin;
Trehalose;
E-cadherin;
Cryopreservation
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2008;14(3):197-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of E-cadherin expression in human skin after cryopreservation. Methods The skin tissue was treated with DMSO-propyleneglycol (TD group) and trehalose-DMSO (DP group), respectively, and cryopreserved in liquid nitrogen (-196 ℃) for 7 and 14 days and the changes after cryopreservation were compared with that of fresh skin (control group). Immunohistochemical staining was used to detect the expression of E-cadherin in the skin.Results Compared with the fresh skin, TD group showed good preservation of the skin, and the expression level of E-cadherin was similar to that in the control group. Conclusion E-cadherin is well preserved in the TD group. Therefore, trehalose-DMSO is an effective cryoprotectant for human skin, and much better than that of traditional low-temperature protectants.
