Expression of endostatin in rat peritoneum and its association with peritoneal neoangiogensis
10.3760/cma.j.issn.1001-7097.2009.06.010
- VernacularTitle:内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系
- Author:
Zhanzheng ZHAO
;
Zhangsuo LIU
;
Yan LI
;
Dan GAO
;
Xianhui LIANG
- Publication Type:Journal Article
- Keywords:
Endostatins;
Peritoneal dialysis;
Neovascularization pathological;
Microvessel density
- From:
Chinese Journal of Nephrology
2009;25(6):437-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of endostatin (ES) in rat peritoneum and its association with peritoneal neoangiogensis. Methods Thirty-two male SD rats were randomly divided into 4 groups: normal control rats (C group), renal failure without PD rats (non-PD group), rats dialyzed with 1.5% PD solution (1.5% PD group) and 4.25% PD solution (4.25% PD group). After regular PD for 28 days, mRNA and protein expression of ES in peritoneal tissues of each group were detected by RT-PCR and immunohistochemistry respectively. Microvessel density (MVD) of peritoneal tissue was assessed using immunohistochemistry with CD34 monoclonal antibody. Results ES mRNA was expressed in each group, 0.47±0.05 in C group, 0.45±0.04 in non-PD group, 0.46±0.04 in 1.5%PD group, 0.47±0.03 in 4.25%PD group, and no significant differences were found among groups. Score of ES protein expression was O in C group, 2 in non-PD group, 4 in 1.5%PD group, and 9 in 4.25%PD group. MVD was 3.13±1.13 in C group, 5.13±1.14 in non-PD group, 9.00±1.51 in 1.5%PD group, 10.75±1.83 in 4.25%PD group, and significant differences were found among groups. Conclusion Uremia circumstance and non-physiological compatibility peritoneal dialysate can increase ES protein expression and MVD, which may participate in and have effects on the course of peritoneal neoangiogensis.
