Effect of syndecan-4 on the proliferation and extracellular matrix secretion of human mesangial cells stimulated by basic fibroblast growth factor
10.3760/cma.j.issn.1001-7097.2009.05.014
- VernacularTitle:syndecan-4对碱性成纤维细胞生长因子诱导人肾小球系膜细胞增殖及细胞外基质分泌的影响
- Author:
Dexuan WANG
;
Qing YANG
;
Ruixia LIN
;
Changlin MEI
;
Bing DAI
;
Jiangqin LIU
;
Lili FU
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor 2;
Mesangial cells;
Protein kinase C-alpha;
Syndecan-4;
Extracellular matrix
- From:
Chinese Journal of Nephrology
2009;25(5):375-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of syndecan-4 on the proliferation and extracellular matrix (ECM) secretion of human mesangial cells(HMC) stimulated by basic fibroblast growth factor (bFGF) and to evaluate the role of syndecan-4-PKCα pathway. Methods The expression of syndecan-4 in HMC was observed by immunofluorescence. After the down-regulation of syndecan-4 in HMC by RNA interference, the cell proliferation was detected by MTT. The secretion of fibronectin (FIN), type IV collagen, type Ⅰ collagen was assessed by ELISA. The copy number of syndecan-4 and PKCα was measured by fluorescent quantitation PCR at different time points. Results Syndecan-4 was expressed in HMC. bFGF could promote the cell proliferation and ECM secretion together with the PKCα copy number per million house-keeping genes of HMC, which could be reversed by the syndecan-4 siRNA transfection (MtT: 48-60 h, P<0.01; FiN: 24 h, P<0.01, 48-96 h, P<0.05; type Ⅳ collagen: 72-96 h, P<0.05; PKCa: 0 h, P<0.05, 12-48 h, P< 0.01). Conclusion Syndecan-4 may regulate the proliferation and ECM secretion of HMC stimulated by bFGF through syndecan-4-PKCα pathway.
