Mechanism of TWEAK on the synthesis of MMP-9 in fibroblast-like synoviocytes of rheumatoid arthritis

VernacularTitle:TWEAK诱导成纤维样滑膜细胞合成MMP-9的研究
Author: Liping XIA ; Jing LU ; Weiguo XIAO
Publication Type:Journal Article
Keywords: Arthritis,rheumatoid; TWEAK; Fibroblast-like synoviocyte; Matrix metalloprotein-ascs 9; p38MAPK
From: Chinese Journal of Microbiology and Immunology 2009;29(7):581-585
CountryChina
Language:Chinese
Abstract: Objective To discuss the role of p38MAPK signal pathway in the process of TWEAK inducing rheumatoid arthritis(RA) fibroblast-like synoviocyte(FLS) to synthesize MMP-9 and look for a new target for RA treatment. Methods RA FLS were primarily cultured and stimulated with TWEAK. Western blot was used to detect the expression of p-p38MAPK and p65 in RA FLS. FLS were pretreated by SB203580 or not. ELISA was used to detect the concentration of MMP-9 in cell-cultured fluid. The mRNA expression of MMP-9 was measured by RT-PCR. Results TWEAK( 100 ng/ml) can make p38MAPK phosphorylated and increased the expression of p65 protein in the cell nucleus. SB203580 can partially inhibit the expression of MMP-9 and MMP-9 mRNA produced by RA FLS which is induced by TWEAK. Conclusion TWEAK induced RA FLS to synthesize MMP-9, in that process, the p38MAPK signal trausduction pathway was in active state, and induced the expression of NF-κB.