The synthesis and shedding of transmembrane CXCL16 in first-trimester human trophoblast
10.3760/cma.j.issn.0254-5101.2009.07.002
- VernacularTitle:早孕滋养细胞膜型及可溶性趋化因子CXCL16表达调控
- Author:
Yu HUANG
;
Yutao LIU
;
Jianhua MA
;
Jie YU
;
Zhumei CUI
;
Dajin LI
- Publication Type:Journal Article
- Keywords:
Trophoblast;
Chemokine;
CXCL16;
Cytokine;
ADAM10
- From:
Chinese Journal of Microbiology and Immunology
2009;29(7):586-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the regulation of CXCL16 synthesis and shedding in first-trimester human trophoblasts. Methods Firstly, we analyzed the expression and secretion of chemokine CXCL16 in primary cultured trophoblasts by immunochemical staining and ELISA. Then we determined the soluble and cell-associated CXCLI6 respectively with and without treatments of cytokine IFN-γ, TNF-α, IL-4 and ADAM10 by ELISA. Results Trophoblast expressed and secreted CXCL16 in a stable level. Cytokine IFN-γ induced both synthesis and secretion of CXCL16 significantly ( P <0. 01 ) in trophoblasts. ADAM10 increased the shedding of chemokine domain of CXCL16 from trophoblasts but didn't influence the synthesis of CXCL16 protein in trophoblast. Conclusion IFN-γ and ADAM10 play important roles in production and shedding of transmembrane CXCL16 in first-trimester trophublasts.
