Purification of native F1 antigen from Yersinia pestis EV76 anti its efficacy against Yersinia pestis in mice
10.3760/cma.j.issn.0254-5101.2009.07.006
- VernacularTitle:鼠疫耶尔森菌F1抗原的纯化方法改进及其免疫原性的评价
- Author:
Zhizhen QI
;
Haihong ZHAO
;
Ruixia DAI
;
Qingwen ZHANG
;
Lingling REN
;
Yonghai YANG
;
Cunxiang LI
;
Hailian WU
;
Jian HE
;
Rongjie WEI
;
Hu WANG
;
Ruifu YANG
;
Zuyun WANG
;
Xiaoyi WANG
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
FI antigen;
Protective immunity
- From:
Chinese Journal of Microbiology and Immunology
2009;29(7):602-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify native F1 antigen from E pestis EV76 strain and determine its ef-ficacy against Y. pestis. Methods A new purification method was developed by the substitution of physical disruption ( glass beads) for organic solvent ( acetone and toluene) one, followed by a combination of ammo-nium sulfate fractionation and SephacrylS-200HR column filtration chromatography. Groups of mice were im-munized with F1 antigen adsorbed to 25% aluminum hydroxide in PBS by intramuscular route. The immu-nized animals were challenged subeutaneously(s, c. ) with 104 CFU of Y. pestis strain 141 at 18 weeks after the primary immunization. Results There was no IgG titre difference between two groups of mice with one-dose immunization, whereas in the two-dose immunization groups, the group F1-40 μg induced a statistically higher antibody titre than the group F1-20 μg. Complete protection was observed for animals immunized with purified F1 antigen by s.c. route. In contrast, the control mice immunized with aluminum hydroxide suc-cumbed to a same dose of Y. pestis 141 challenge. Conclusion This purification strategy is a simple and ef-fective, and can be operated in a large scale. Native F1 antigen extracted from Y. pestis EV76 is highly im-munagenic, and can be used as a key antigen component to develop sub-unit vaccine of plague.
