Pro-inflammatory effect of transforming growth factor β1 in rat peritoneal mesothelial cells
10.3760/cma.j.issn.1001-7097.2009.06.006
- VernacularTitle:转化生长因子β1在大鼠腹膜间皮细胞中的促炎作用
- Author:
Ling YE
;
Weiying CHEN
;
Xin WANG
;
Xiaoyau LI
;
Xueqing TANG
;
Xueqing YU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
p38 mitogen-activated protein kinases;
Monocyte chemoattractant protein !;
Smad7
- From:
Chinese Journal of Nephrology
2009;25(6):420-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pro-inflammatory effect of transforming growth factor β1 (TGF-β1) in rat peritoneal mesothelial cells (RPMCs) and its machanism. Methods TGF-β1-induced RPMCs model in vitro was established, and the expression of MCP-1 in the TGF-β1-induced RPMCs was observed. The intervention of Smad7 on the expression of MCP-1 and p38 signal proteins induced by TGF-β1 in RPMCs was explored as well as the intervention of p38 inhibitor SB203580 on the expression of MCP-1 induced by TGF-β1 in RPMCs. Results TGF-β1 could stimulate MCP-1 expression in RPMCs. Compared with control group, MCP-1 mRNA levels were significantly increased after 3 h treatment with TGF-β1 (P<0.05), peak MCP-1 induction occurred at 6 h (P<0.01), and the stimulatory effect of TGF-β1 persisted through 24 h (P<0.05). MCP-1 protein levels were significantly increased after 6 h treatment with TGF-β1(P<0.05), peak MCP-1 induction occurred at 48 h(P<0.01). Over-expressed Smad7 and p38 inhibitor could reduce the expression of MCP-1 induced by TGF-β1 (P<0.05). TGF-β1 could activate p38 signaling pathway, but over-expressed Smad7 could inhibit this role of TGF-β1. Compared with control group, the expression level of p-p38 was increased in TGF-β1-stimulated group. Compared with TGF-β1-stimulated group, the expression level of p-p38 was reduced in Smad7 gene transfer group. Conclusions TGF-β1-induced MCP-1 expression in rat peritoneal mesothelial cells is p38MAPK dependent.
