Sequence analysis of the HA gene of influenza virus (H1N1) in Shenzhen from 1995 to 2007

VernacularTitle:1995-2007年深圳市H1N1流感病毒血凝素基因的序列分析
Author: Xing Lü ; Xiaowen CHENG ; Chunli WU ; Jianfan HE ; Shisong FANG
Publication Type:Journal Article
Keywords: H1N1; HA gene; Sequence analysis
From: Chinese Journal of Microbiology and Immunology 2009;29(7):627-630
CountryChina
Language:Chinese
Abstract: Objective To analyze the genetic characteristics of H1N1 influenza viruses isolated in Shenzhen during 1995 to 2007. Methods The hemagglutinin(HA) gene of these viruses were sequenced. Phylogenetic analysis of the sequences was performed with Simmonic and Mega software. Results The H1N1 influenza viruses isolated in Shenzhen from 1995 to 2007 were divided into chide A, B and C. Some viruses from 2005 to 2006 clustered in the same group with the viruses of 2001. Furthermore, some of the vaccine strains recommended by WHO were found lagged behind the strains isolated in Shenzhen. Some mu-tations occurred on the antigenic sites as well as receptor-binding site(RBS). Except the viruses of 1995, the other viruses had deleted at the site 137. Conclusion Characterization of the HA gene revealed that most of the amino acid substitutions occurred on the antigenic sites and RBS. Furthermore, it was discovered that the mutations occurred on different antigenic regions in different years.