Detection for respiratory viruses in children with multiplex PCR
10.3760/cma.J.issn.0254-5101.2009.07.021
- VernacularTitle:多重PCR检测儿童呼吸道感染常见病毒
- Author:
Lie HUANG
;
Fansheng ZENG
;
Qiong WANG
;
Runxiang WU
;
Xiaofang LUO
;
Xuedong LU
- Publication Type:Journal Article
- Keywords:
Respiratory virus;
Multiplex PCR
- From:
Chinese Journal of Microbiology and Immunology
2009;29(7):664-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the prevalence and occurrence of respiratory viruses in chil-dren in Southern China. Methods Respiratory virus were identified from nasopharyngeal aspirates and throat swabs collected from children with bronchiolitis, bronchopneumonia and asthmatic bronchitis who visi-ted the Pediatrics of Affiliated Hospital to Shantou University, Shenzhen Fourth People's Hospital, Jieyang People's Hospital, during the period of June 2006 to June 2008. Respiratory virus was detected by multiplex PCR. Results Viruses were detected in 362 patients(52.77% ), among them, RSV infection was the most frequent, 31.22% of 113 patients. RHV was found in 16.85% (61 patients), IVA in 14.36% (52 pa-tients), ADV in 9.67% (35 patients), PIV in 16. 02% (58 patients), hBOV in 6. 08% (22 patients), hMPV in 4.97% ( 18 patients) and IVB in 0. 83% (3 patients). Conclusion The data indicate that RSV, RHV and IVA is an important etiological agent for respiratory infections in children during the survey period. RSV, IVA combined other virus are the most virus for combined infection, and the manages are worked out by the doctor for the diagnosis and treatment depended on the detected results of the pathogen.
