Feasibility study of differentiation of invitro induced rat bone marrow-derived mesenchymal stem cells into retinal pigment epithelial cells

VernacularTitle:体外诱导大鼠骨髓间充质干细胞向视网膜色素上皮细胞分化的可行性研究
Author: Fei GAO ; Fangtian DONG
Publication Type:Journal Article
Keywords: Stem cells/cytology; Myeloid progenitor cells/immunology; Cell culture techniques/methods; Cell differentiation/immunology; Pigment Epithelium of eye/cytology; Animals,laboratory
From: Chinese Journal of Ocular Fundus Diseases 2009;25(4):254-256
CountryChina
Language:Chinese
Abstract: Objective To investigate the feasibility of differentiation of invitro induced rat bone marrow-derived mesenchymal stem cells(rMSCs) into retinal pigment epithelial (RPE) cells.Methods The rMSCs from Brwon-Norway (BN) rats were isolated and cultured by adherent screening method.RPE cells lysate made by repeated freeze-thawing was put into the rMSCs culture system to identify whether the induced cells could express characteristic label cytokeratin(CK)and S-100 simultaneously or not.Results The growth rate of rMSCs induced by RPE cells lysate was slower and protuberant burr surrounded the fusiform cells.The results of immunoblotting and double immunofluorescence showed that partial induced cells expressed CK and S-100 simultaneously.The result of flow cytometry indicated that 14.1% induced cells expressed CK and S-100 simultaneously.Conclusion Induced by RPE cells lysate,rMSCs can differentiate into RPE cells.