The effect of meningococcal serogroup A polysaccharide-protein conjugate vaccine prepared with TT and rCTB on mucosal immune responses
10.3760/cma.j.issn.0254-5101.2009.06.011
- VernacularTitle:rCTB和TT为蛋白载体的A群流脑多糖黏膜免疫初步研究
- Author:
Yanyan GAO
;
Ring ZHANG
;
Xuefeng ZHANG
;
Yuqin JIN
;
Xinliang SHEN
;
Qiming LI
- Publication Type:Journal Article
- Keywords:
Polysaccharide-protein conjugate vaccine;
Protein carrier;
Tetanus toxoid(TT);
Re-combinant cholera toxin B subunit (rCTB)
- From:
Chinese Journal of Microbiology and Immunology
2009;29(6):522-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the feasibility of the recombinant cholera toxin B subunit (rCTB) as a carrier protein candidate for the preparing of polysaccharide-protein conjugate, and to discuss the immune effects of tetanus toxoid (TT) as the carrier protein in mucosal delivery vaccine. Methods The refolded pentrumer protein, rCTB was obtained by genetic engineering methods. Then conjugated the refold-ed protein with group A meningococcal polysaccharide (GAMP) using the chemical method(ADH) ,the pol-ysaccharide-protein conjugates(GAMP-rCTB) were prepared. BALB/c mice were immunized either intraper-itoneally ( i. p. ) or intranasally ( i. n. ) with GAMP-rCTB. Moreover, GAMP-TT vaccine that TT as carrier proteins was i.n. immunized to the mice. The evaluation of immunology is performed. Results The conju-gates of polysaccharide-potein with the rCTB and TT as protein carrier both are able to elicit high level of GAMP specific IgG antibody in serum after i.n. immunization, and the conjugates can also elicit specific IgA antibody in lung lavage and intestinal mucosa. Conclusion rCTB and TT can both as the protein carri-er for polysaccharide-protein conjugate as mucosal vaccine. The route of intranasal may be more ways for im-mune function than i.p. immunization when rCTB is used as the carrier of the polysaccharide-protein conju-gates.
