Effect of mycophenolate mofetil on matrix metalloproteinase-9 and transforming growth factor β1 expression in the kidneys of type 2 diabetic rats
10.3760/cma.j.issn.1001-7097.2009.07.014
- VernacularTitle:霉酚酸酯对糖尿病大鼠肾脏基质金属蛋白酶9、转化生长因子β1表达的影响
- Author:
Yan YAN
;
Qinkai CHEN
;
Yu WANG
;
Ying WANG
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Matrix metalloproteinase 9;
Transforming growth factor beta 1;
Mycophenolate mofetil
- From:
Chinese Journal of Nephrology
2009;25(7):543-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effects of mycophenolate mofetil (MMF) on the kidneys of diabetic rats and elucidate the associated mechanisms. Methods Wistar rats were divided into three groups: normal control rats, diabetic rats, and diabetic rats received and blood glucose were measured, and kidney pathology was observed. Inmmunohistochemistry and RT-PCR were used to analyze the expression of matrix metaUoproteinase-9 (MMP-9) and transforming growth factor 151 (TGF-β1). Results As compared with normal control rats, the 24 h urinary albumin excretion [(26.80±0.82) mg vs (6.64±1.42) mg], blood glucose[(22.18±3.36)mmol/L vs (6.40±0.87) mmol/L], Ccr [(0.220±0.380) ml/min vs (0.098±0.015) ml/min] of the diabetic rats were rised remarkbably. The 24 h urinary albumin excretion [(16.17±1.15) mg] and Ccr [(0.207±0.377) ml/min] of the diabetic rats received MMF were lower as compared to diabetic rats (P<0.05). MMP-9 in renal tissue of normal control rats was mainly expressed in glomerular mesangial ceils and renal tubular epithelial cells. Such MMP-9 expression was weak in diabetic rats and improved in the diabetic rats received MMF. There were significant differences among 3 groups. The expression of TGF-β1 was on the contrary. Conclusion Mycophenolate mofetil decreases 24 h urinary albumin, Cer and glomerular volume, which may be associated with the increase of MMP-9, the decrease of TGF-β1 expression and extracellular matrix deposition in renal tlssue.
