The levels of transforming growth factor-β1 mRNA and estrogen receptor βwt mRNA expression on peripheral blood monocytes of patients with chronic fatigue syndrome
10.3760/cma j.issn.1009-9158.2009.07.005
- VernacularTitle:慢性疲劳综合征患者外周血单个核细胞中转化生长因子β1 mRNA及雌二醇受体β野生型mRNA的表达水平
- Author:
Haiying ZHANG
;
Zhandong LIU
;
Chaojun HU
;
Yang ZHANG
;
Xue FENG
;
Shumeng BAO
;
Yongbo ZHANG
;
Dexin WANG
;
Yongzhe LI
- Publication Type:Journal Article
- Keywords:
Fatigue syndrome,chronic;
Leukocytes,mononuclear;
Transforming growth factor
- From:
Chinese Journal of Laboratory Medicine
2009;32(7):745-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of TGF-β1 mRNA and ERβwt mRNA in peripheral blood monoanclear cells (PBMCs) in patients with chronic fatigue syndrome (CFS), and their relationship with pathogenesis of CFS. Methods Fluorescence quantitative RT-PCR (FQ-RT-PCR) was used to examine TGF-β1 mRNA and ERβwt mRNA expression of peripheral blood monocytes in 63 cases with CFS,50 cases with other diseases, and 50 healthy controls. The gene expression levels were calculated with the formula △Ct=Ct(target gene) - Ct(internal control). Results The mean TGF-β1 mRNA expression of CFS patients (△Ct = 3.27 ± 0. 58) was higher than that of disease controls (△Ct = 4. 54 ± 1.05, t = 8. 11, P <0.01) and that of healthy controls (△Ct = 4. 37 ± 1.00, t = 7. 02, P < 0. 01). The mean ERβwt mRNA expression of CFS patients (△Ct =9. 34 ±0. 92) was lower than that of disease controls(△Ct =7.12±0. 47, t = 15.44 ,P < 0. 01) and that of healthy controls(△Ct = 7. 10 ±0. 48, t = 15.47, P < 0. 01). Conclusions The TGF-β1 mRNA and ER βwt mRNA expression levels of PBMCs are siguificantly elevated in patients with CFS. It may be implicated in the pathogenesis of CSF.
