The role of PDK in regulating cytokine secretion by extracellular matrixes
10.3760/cma.j.issn.0254-5101.2009.07.005
- VernacularTitle:P13K在细胞外基质分泌细胞因子表达中的作用
- Author:
Jin LIU
;
Shanna SHI
;
Shuyun XU
;
Yongjian XU
;
Jie XU
;
Zhongren CHEN
;
Zhengyan WANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Extracellular matrixes;
Airway smooth muscle cells;
Immunomodulatory function;
Phesphoinasitide 3-kinase
- From:
Chinese Journal of Microbiology and Immunology
2009;29(7):597-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects of extracellular matrixes, including fi-bronectin(FN), and collagen Ⅰ (ColⅠ ) on the immunologic function of human airway smooth muscle cells (HASMCs) passively sensitized with asthmatic serum, and the role of phosphoinosifide 3-kinase (PI3K). Methods Primarily cultured HASMCs were inoculated on the blank plates or on the plates coated with difference matrix proteins, added 10% asthmatic serum to passively sensitized non-asthmatic HASMCs and 10% non-asthmatic serum treated HASMCs as control, cell pretreated with PI3K inhibitor LY294002 for 30 min. The expressions of RANTES, Eotaxin, TGF-β1 mRNA were observed by RT-PCR and RANTES, Eotaxin, TGF-β1 protein in the ceil culture supernatunts was detected by enzyme-linked immtmosorbent assay (ELISA). Results Compared with the control serum group, the expressions of RANTES, Eotaxin, TGF-β1 mRNA of HASMCs and those protein in HASMCs culture supematants were significantly increased in the asthmatic serum group and the control serum + FN group and the control serum + Col Ⅰ group ( P < 0.05 ). The expressions of RANTES, Eotaxin, TGF-β1 mRNA of HASMCs and protein in HASMCs culture superna-tants were significantly increased in the asthmatic serum + FN group and the asthmatic serum + Col Ⅰ group. 50 μmoL/L LY294002 could significantly inhibit the expressions of RANTES, Eotaxin, TGF-β1 mRNA of HASMCs and protein in HASMCs culture supematants(P < 0.05). Conclusion These results suggest ex-tracellular matrixe may regulate immunomodulatory function of HASMCs passively sensitized with asthmatic serum and PI3K signaling pathway may play an important role in the process.
