Clinical significance of blood micrometastasis in pN0 esophageal squamous cell carcinoma
10.3760/cma.j.issn.1006-9801.2009.07.006
- VernacularTitle:pN0期食管鳞癌血液微转移检测的临床意义
- Author:
Chaowei TANG
;
Chunli WANG
;
Shuangping ZHANG
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Neoplasms,squamous cell;
Neoplasms metastasis;
Real-time fluorescent quantitative RT-PCR;
MMP-7;
hTERT mRNA
- From:
Cancer Research and Clinic
2009;21(7):450-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance and the impact on prognosis of blood micrometastasis in the patients with pN0 esophageal squamous cell carcinoma. Methods Total RNA was extracted with TRIzol and mRNA was transcribed reversely into cDNA. RT-PCR was used to detect MMP-7 mRNA and hTERT mRNA in blood. △△Ct sample values were calculated with post-operative follow-up of 3 month, 6 month, 12 month. Results Statistical results suggested that blood micrometastasis was related to differentiation grade and pTNM staging (P=0.000, P=0.000 respectively), but not to age, sex, length of turnout (P0.05). Follow-up results suggested that the degree of invasion and tumor metastasis (recurrence) was no correlation; follow-up to 6 month and 12 month, tumor metastasis (recurrence) was associated with blood micrometastasis, and follow-up to 12 month, compared with the tumor metastasis (recurrence) probability of blood micrometastasis-positive patients and negative patients, the former was as 6.44 times as the latter. (OR=6.440, 95 % CI 1.547-26.822). Conclusion Blood micrometastasis testing is of great significance to early diagnosis and prognosis judgment in pN0 esophageal squamous cell carcinoma patients.
