Effect of high glucose on the expression of liver X receptors and ABCA1 in human THP-1 macrophages

VernacularTitle:高糖刺激对人THP-1巨噬细胞肝X受体和三磷酸腺苷结合盒转运子A1表达的作用
Author: Yixin QIAN ; Zhaohui NI ; Leyi GU ; Huili DAI ; Yucheng YAN ; Weiming ZHANG ; Ling WANG ; Zanzhe YU ; Qingwei WU ; Jiaqi QIAN
Publication Type:Journal Article
Keywords: Diabetes mellitus; Arteriosclerosis; Liver X receptor; ATP-binding cassette transporter A1
From: Chinese Journal of Nephrology 2009;25(8):630-634
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of high glucose on the expression of liver X receptors (LXRs) and ATP-binding cassette transporter A1 (ABCA1) in human macrophages (THP-1 cell line). Methods THP-1 monocytes were differentiated into macrophages by induction of phorbol 12, 13-dibutyrate (PMA). Surface markers of macrophages were identified by CD68 immunohistochemistry. The macrophages were cultured with different concentration (5.6, 11.1, 22.2 and 33.3 mmol/L) of glucose and different time (0, 0.5, 2, 6, 12, 24, 48, 72 h). Real time PCR and Western blotting methods were used to examine the mRNA and protein expression of LXRs and ABCA1. Results As compared to 5.6 mmol/L glucose, macrophage LXRβ and ABCA1 were decreased significantly at both mRNA and protein levels in dose-and time-dependent manner (P<0.05). Conclusion Hyperglycemia may play a role in the pathogenesis of arteriosclerosis through the inhibition of LXRs and ABCA1 expression in diabetic patients.