Quantification and genotyping of PCV2 DNA in the tissues of PCV2-infected conventional pigs with different clinical signs.
- Author:
Hye Kwon KIM
1
;
Yuzi LUO
;
Hyoung Joon MOON
;
Seong Jun PARK
;
Se Mi RHO
;
Jae Yeon HAN
;
Van Giap NGUYEN
;
Bong Kyun PARK
Author Information
1. College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. parkx026@snu.ac.kr
- Publication Type:Original Article
- Keywords:
clinical sign;
genotype;
PCV2a;
PCV2b;
Quantification
- MeSH:
Circovirus;
Coinfection;
Cross-Sectional Studies;
DNA;
Genotype;
Swine
- From:Korean Journal of Veterinary Research
2011;51(1):7-14
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was focused on the genotyping and quantification of Porcine circovirus type 2 (PCV2) in thirty PCV2-positive pigs with different clinical symptoms (PCV2-infected without wasting, PCV2-infected with wasting, PCV2-infected with wasting and lymphoid depletion). The quantity of PCV2 DNA in diverse tissues was significantly differed among these groups. (One-way ANOVA test, p < 0.001) Interestingly, PCV2-DNA load in tissues of PCV2-infected pigs without wasting and PCV2-infected pigs with wasting and lymphoid depletion were not significantly differed (p = 0.38), while they were all significantly higher when compared with PCV2-infected pigs with wasting-only. PCV2 DNA quantity in tissues was significantly higher in PCV2a and 2b co-infected pigs compared to the PCV2b only-infected pigs (Wilcoxon test, p = 0.039). The PCV2a and 2b co-infected pigs had increased wasting and lymphoid depletion rate but it was not statistically significant. Therefore, this cross-sectional study suggested that PCV2 DNA load in tissues was diverse by clinical and histological findings. Furthermore, co-infection of PCV2a and 2b affected to the PCV2 DNA load in tissues with increased rate of wasting and lymphoid depletion.
