Impact of pentoxifylline on the pathway of high glucose-induced expression of connective tissue growth factor in mesangial cells
10.3760/cma.j.issn.1001-7097.2009.08.011
- VernacularTitle:己酮可可碱对高糖诱导肾小球系膜细胞结缔组织生长因子表达信号通路的影响
- Author:
Jingyun ZHANG
;
Demin YU
;
Chunjun LI
;
Mingzhen LI
;
Qiumei ZHANG
;
Pei YU
- Publication Type:Journal Article
- Keywords:
Pentoxifylline;
Mesangial cells;
Transforming growth factor beta;
Connective tissue growth factor
- From:
Chinese Journal of Nephrology
2009;25(8):640-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pentoxifyccine (FIX) on the pathway of high glucosd-induced expression of CTGF in mesangial cells. Methods Cultured rat mesangial celld were used to study the influence of different concentration of high glucose on the expression of TGF-β, CTGF, p-Smad2/3, Smad7 and FN in different exposure time. Furthermore the effect of high glucose plus TGF-β neutral antibody and different concentration of FIX on the obove expression was evaluated as well. Results High glucose could increase TGF-β, CTGF mRNA and protein expression in mesangial cells (P<0.05) in time-and dose-dependent manner, and at the same time p-Smad2/3 expression increased and Smad7 expression decreased (P<0.05).The blockage of TGF-β could decrease high glucose-induced CTGF mRNA and protein expression by 86.4% and 91.8%. PTX could suppress high glucose-induced CTGF expression in mesangial cells. When the PTX dosage increased, the suppressive effect became more remarkable, but PTX had no influence on the TGF-β expression. Conclusions High glucose up-regulates CTGF mRNA and protein expression mainly through TGF-β-Smads pathway. PTX can suppress CTGF expression effectively, but has no direct inhibition of TGF-β expression.
