STR profiling on authentication of human cell lines for production
10.3760/cma.j.issn.0254-5101.2009.07.015
- VernacularTitle:STR图谱用于生物制品生产用人源细胞鉴别的研究
- Author:
Shufang MENG
;
Yu WU
;
Jianping FENG
;
Xueling WU
;
Youchun WANG
;
Defu LI
- Publication Type:Journal Article
- Keywords:
Cell identification;
STR profiling;
Human diploid cell strains;
293 cell lines
- From:
Chinese Journal of Microbiology and Immunology
2009;29(7):636-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the application of short tandem repeat (STR) profiling in quality control of human cell lines used for biological production. Methods The methods detecting 9 and 16 STR loci to identify human cell lines by PCR-capiilary electrophoresis were established respectively. Human cell lines, which were derived from many corporations and including diploid cell strains used for virus-vaccine production and 293 cell lines used for gene therapy products, were analyzed and compared by these two methods. Results The STR profiling methods used for authentication of human cell lines were established. Most of human diploid cell strains(20/21 ) used for virus-vaccine production from 13 corporations were iden-tiffed as the intended cells and no cross-contamination was found. However, one MRC-5 cells was identified as a false cell line and one MRC-5 had 3 alleles in D13S317 locus. For 12 strains of 293 cell lines, there were significant differences in STR profiling from different manufactures, which was likely be explained that the sources and gene modifications of these 293 cell lines are not well known and their genes are unstable during passage. Conclusion The STR profiling method has the advantages of high sensitivity and specifici-ty, and can be used for authentication of each of human cell lines for biological production.
