The virulence of Brucella abortus isolated from cattle in Korea.
- Author:
Jeong Ju LIM
1
;
Jeong Hwa KIM
;
Dong Hyeok KIM
;
Jin Ju LEE
;
Dae Geun KIM
;
Moo Hyung JUN
;
Sang Hun KIM
;
Hong Hee CHANG
;
Hu Jang LEE
;
Won Gi MIN
;
Suk KIM
Author Information
1. College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. kimsuk@gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Brucella abortus;
cattle;
phagocytes;
virulence
- MeSH:
Agglutination Tests;
Animals;
Brucella;
Brucella abortus;
Cattle;
Korea;
Macrophages;
Membrane Proteins;
Mice;
Phagocytes;
Polymerase Chain Reaction;
Sprains and Strains
- From:Korean Journal of Veterinary Research
2011;51(1):15-20
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class I, lower internalization than that of B. abortus 544; class II, similar internalization to that of that of B. abortus 544; class III, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class III showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.
